Courtesy: ISO/TS 28037:2010 Determination and use of straight-line calibration functions The Bradford assay is a colorimetric assay that measures protein concentration. The reagent Coomassie brilliant blue turns blue when it binds to arginine and aromatic amino acids present in proteins, thus increasing the absorbance of the sample. The absorbance is measured using a spectrophotometer, at the maximum absorbance frequency (Amax) of the blue dye (which is 595 nm). In […]
Category Archives: ISO/TS 28037:2010 determination and use of straight-line calibration functins
Courtesy: ISO/TS 28037:2010 determination and use of straight-line calibration functions In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. A calibration curve is one approach to the problem […]
Courtesy: ISO/TS 28037:2010 determination and use of straight-line calibration functions ISO/TS 28037:2010 is concerned with linear, that is, straight-line, calibration functions that describe the relationship between two variables X and Y, namely, functions of the form Y = A + BX. Although many of the principles apply to more general types of calibration function, the approaches described exploit the simple form of the […]